Use of an Innovative Silage of Agro-Industrial Waste By-Products in Pig Nutrition: A Pilot Study of Its Effects on the Pig Gastrointestinal Microbiota

The aim of this study was to evaluate whether dietary supplementation with an innovative silage (IS) created using 60% olive mill waste, 20% grape pomace, and deproteinised feta cheese waste solids can modulate the composition intestinal microbiota in weaned (Exp. 1) finishing 2) pigs. In Exp. 1 (40 day supplementation), forty-five crossbred pigs were randomly assigned 0% (Control), 5%, or 10% IS groups (15 replicates/experimental diet). 2 (60 eighteen from fed control diet for 8 weeks before being re-assigned their original experimental diets (six Performance parameters recorded. Ileal caecal digesta mucosa collected at end each experiment analysis 16S rRNA gene sequencing (five pigs/experimental six 2). No significant effects on pig growth observed both experiments. 1, 5% increased relative abundance Prevotellaceae family, Coprococcus genus, Alloprevotella rava (OTU_48) reduced Lactobacillus genus caecum compared and/or (p < 0.05). 2, led compositionally more diverse different ileal group 0.05; p = 0.066 β-diversity ileum). Supplementation Clostridium celatum/disporicum/saudiense (OTU_3) ileum Bifidobacterium pseudolongum (OTU_17) Streptococcus gallolyticus/alactolyticus (OTU_2) Similar C. S. has potential beneficially alter gastrointestinal